An autoclave is a commonly used piece of equipment in biomedical laboratories. Autoclaves pose many hazards including physical hazards (e.g. heat, steam and pressure) and biological hazards. These guidelines are governed by ADEM: Alabama Department of Environmental Management “Solid Waste Disposal Act” 335-13-7-08, infectious waste treatment regulations, CDC: Centers for Disease Control–Biosafety in Microbiological and Biomedical Laboratories (BMBL) and UA’s Biological Safety Manual. These guidelines are intended to provide practical information that can be utilized by all researchers to safely operate the autoclaves at UA. Individual labs are encouraged to use this document as a guide for training new personnel on the safe use of autoclaves.